Alpha Amylase Biochemistry Test Kit
Athenese DX Alpha Amylase Biochemistry Test Kit
The TRUEchemie α – Amylase (Alpha amylase) test kit is for the quantitative determination of Amylase concentration in human serum or plasma.
- Ready-to-use, single reagent
- Linearity up to 2000 U/L
- Bilirubin (mixed isomers) < 10 % interference up to 600 µmol/L, haemolysis < 10 % interference up to 5 g/L and lipemia < 10 % interference up to 5 g/L
- Factor or calibrator-based assay
The direct amylase assay involves the use of a chromogenic substrate CNPG3 (2-chloro-4nitrophenyl linked with Galactomaltoside) which acts upon α – Amylase to release more than 95% of 2-chloro-4-nitrophenol (CNP), and form 2-chloro-4-nitrophenyl-α-D-maltoside (CNPG2), maltotriose (G3) and Glucose (G). The rate of formation of 2-chloro-4-nitrophenol is proportional to the α – Amylase activity in the sample, which can be monitored by kinetic assay at 405 nm. α- Amylase 10 CNPG3, 9CNP + CNPG2 + 9G3 +G
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