Athenese DX Bilirubin Direct Biochemistry Test Kit

The TRUEchemie Bilirubin – Direct Test Kit for the quantitative determination of direct and total bilirubin in human serum.

• Reliable
• Accurate
• Cost effective
• Preferred by lab technician worldwide

PRINCIPLE

Sulfanilic acid reacts with sodium nitrite to form diazotized Sulfanilic acid. In the presence of accelerator (cetrimide), conjugated and unconjugated bilirubin reacts with diazotized Sulfanilic acid to form azobilirubin (Bilirubin Total 4+1). In the absence of accelerator, only conjugated bilirubin reacts (Bilirubin Direct 4+1). The increase of absorbance at 546 nm is proportional to bilirubin concentration Sulfanilic acid + NaNO2   ———> Diazotized Sulfanilic acid Bilirubin + Diazotized Sulfanilic acid —————–> Azobilirubin PACK

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