Dengue Ag (NS1) ELISA Kit

The RecombiLISA Dengue Ag (NS1) ELISA Kit is a solid-phase, enzyme linked immunosorbent assay that was developed using the antibody sandwich technique. The two main kit components are:

1) A 96 breakable well plate with the solid microwells pre-coated with rabbit anti- Pan Dengue NS1 antibody

2) A conjugate solution composed of antibodies against Dengue NS1 antigen conjugated with horseradish peroxidase (HRP-anti-dengue NS1 conjugates)

This test is intended for the qualitative detection of dengue NS1 antigen (DEN1, 2, 3, 4) in human serum or plasma. It is intended to be used by professionals as an aid in the early detection of dengue virus infection . Any interpretation or use of this test result must also rely on other clinical findings as well as on the professional judgment of healthcare providers.


SKU: E0312 Categories: ,

Product Features

  • Analytic sensitivity: Detects recombinant Dengue type 2 NS1 at a level as low as 0.3 ng/mL.
  • Sensitivity: 100%
  • Specificity: 97.8%
  • SeraCare Dengue Early Infection AccuSet Performance Panel: 100% agreement with PanBio Dengue Early ELISA
  • Cross Reactivity: No cross reaction with HCV, Syphilis, HBsAg, ANA, HIV, HAMA, H. pylori, RF, Malaria
  • Hook Effect: No hook effect up to 0.1 mg/mL
  • Interference: No interference with Salicylic acid: 4.34 mmol/L; Glucose: 55 mmol/L; Sodium citrate: 1.3 %;Heparin: 3,000 U/L; Creatinine: 5 mmol/L; Bilirubin: 10 mg/dL; EDTA: 3.4 ?mol/L
  • Intra Assay Precision: CV < 10%
  • Inter Assay Precision: CV < 7%
  • Shelf life: 18 months
  • Open vial stability: 8 weeks at 2-8°C, or until the expiration date

Additional Information

Well Kit

  • Microwells coated with monoclonal anti-dengue Ab, 8 wells x 12 strips
  • Dengue Ag positive control 0.5 mL
  • Dengue Ag negative control 0.5 mL
  • HRP–anti-dengue Conjugate 6mL
  • Wash buffer (30 x concentrate) 20 mL
  • TMB substrate A 6 mL
  • TMB substrate B 6 mL
  • Stop solution 6 mL
  • ELISA Working Sheet 2 sets
  • Product insert 1 set


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