HAV IgM Elisa Test
CTK Biotech, USA HAV IgM ELISA Kit 96 Test
The RecombiLISA HAV IgM ELISA Test is a solid-phase enzyme-linked immunosorbent assay for the qualitative detection of IgM anti-hepatitis A virus in human serum or plasma.
- Utilizes IgM capture technique
- Detects IgM anti-HAV in serum or plasma
- Evaluated with BBI HAV serum conversion and HAV mixed titer performance panels
- Useful for identifying acute HAV infections
- Microwells coated with anti-human IgM antibody
- HAV IgM negative control
- HAV IgM positive control
- HRP-HAV conjugates
- Wash buffer (30 x concentrate)
- TMB substrate A
- TMB substrate B
- Stop solution
- ELISA Working Sheet
- Product insert
- Detection of IgM antibodies in serum, plasma and whole blood specimen
- Comparable performance v.s. EIA: relative sensitivity: 90.6%, relative specificity: 97.6%, overall agreement: 95.4%
- 24 month shelf life at 2-30°C
The RecombiLISA HAV IgM ELISA Test is a solid-phase enzyme-linked immunosorbent assay based on the principle of the IgM capture technique for the detection of anti-HAV IgM in human serum or plasma. The RecombiLISA HAV IgM ELISA Test is composed of two key components: 1) Solid microwells pre-coated with monoclonal anti-human IgM antibody; 2) Liquid conjugates composed of HAV antigens conjugated with horseradish peroxidase (HRP-HAV conjugates). During the assay, the test specimen is first incubated with the coated microwells. The anti-HAV IgM antibodies, if present in the specimen, bind to the antibodies coated on the microwell surface, and any unbound specimen is then removed by a wash step. During a second incubation with the HRP-HAV conjugates, the anti-HAV IgM antibodies absorbed on the surface of microwell react to the HRP-HAV conjugates, forming a conjugate complex. Unbounded conjugates are then removed by washing. After addition of the TMB susbstrate, the presence of the conjugate complex is shown by a blue color resulting from a reaction between the enzyme and substrate. The reaction is then quenched by addition of the Stop Solution and the absorbance value for each microwell is determined using a spectrophotometer at 450/620-690 nm.
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