HbA1c Quanti Card
HbA1c Quanti Card is a rapid and sensitive immunoassay for the quantitative determination of HbA1c (Glycated hemoglobin) in human whole blood with i-Quant Analyzer. The test is used for routine monitoring of long term glycemic status in patient with diabetes mellitus.
PRINCIPLE (ANTIGEN-ANTIBODY REACTION)
HbA1c Quanti Card is based on fluorescence immunoassay technology. The test uses anti- HbA1c antibody and anti-hemoglobin antibody immobilized separately on a nitrocellulose strip. Whole blood sample is added to the mixture of Detection Buffer and Assay Buffer, resulting in hemolysis of red blood cells. The mixture containing HbA1c and Hb from the hemolyzed red blood cells and fluorescence labelled HbA1c and Hb from Detection Buffer, is added to the sample well of the cartridge. The mixture migrates through the nitrocellulose strip by capillary action. When the complex meets the line of the corresponding immobilized antibody on nitrocellulose strip, the fluorescence signal is produced from immuno complex. The signal is interpreted and the result is displayed on i-Quant Analyzer in terms of percentage.
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