GB, Taiwan HEPAVASE MA-96 Elisa Kit 96 Test
HEPAVASE MA-96 (TMB) is an enzyme immunoassay kit for in vitro qualitative detection of IgM antibody to hepatitis A virus (Anti-HAV IgM) in human serum or plasma (heparin, EDTA or citrate).
HEPAVASE MA-96 (TMB) is a solid-phase enzyme immunoassay (ELISA= enzyme-linked immunosorbent assay) – based on the principle of “IgM capture”. The solid phase of the microtiter plate is made of polystyrene wells coated with anti-human IgM, while peroxidase-conjugated Anti-HAV acts as liquid phase. When a serum or plasma specimen containing Anti-HAV IgM is added to the Anti-human IgM-coated wells and incubated, IgM antibodies present in the specimen bind to the Anti-human IgM on the wells. After addition of an HAV Ag-containing solution and a solution containing Peroxidase-conjugated anti-HAV a further incubation takes place, during which (Anti-h IgM) • (Anti-HAV IgM) •(HAV Ag) • (Anti-HAV• Peroxidase) complex is formed on the wells. After washing the microtiter plate to remove unbound material, a solution of TMB substrate is added to the wells and incubated. If Anti-HAV IgM is present in the specimen, after washing, the activity of peroxidase on the wells reflects the content of anti-HAV IgM in a specimen. The peroxidase-TMB reaction is stopped by addition of sulfuric acid. The optical density of developed color is read with a suitable photometer at 450 nm with a selected reference wavelength within 620 to 690 nm*8
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