GB, Taiwan HIVASE 1+2 Elisa Kit 96 Test
The HIVASE 1+2 ELISA Kit is a solid phase enzyme linked immunoabsorbent assay for the qualitative detection of anti-HIV-1 including subtype O and anti-HIV-2 antibodies (including isotype IgG, IgM and IgA) in human serum or plasma.
HIVASE 1+2 ELISA Kit is a solid phase enzyme linked immunoabsorbent assay based on the principle of the double antigen-sandwich technique for the detection of the various antibodies against HIV-1 and/or HIV-2 in human serum or plasma.
The HIVASE 1+2 ELISA is composed of two key components:
1) Solid microwells pre-coated with recombinant HIV-1 and HIV-2 antigens;
2) Liquid conjugates composed of recombinant HIV-1 and HIV-2 antigens conjugated with horse radish peroxidase (HIV 1+2 HRP conjugates).
During the assay, the test specimen is first incubated with the coated microwells. The anti-HIV-1 and anti-HIV-2 antibodies, if present in the specimen, bind to the antigens coated on the microwell surface. In the second incubation with the HRP- HIV 1+2 conjugates, the anti-HIV-1 and antiHIV-1 antibodies absorbed on the surface of microwell react to the HRP-HIV 1+2 conjugates. Unbounded conjugates are then removed by washing. The presence of the complexed conjugates is shown by a blue color upon additional incubation with TMB
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