BIOS, USA Human Insulin Enzyme Immuoassay Elisa Kit 96 Test
Human Insulin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Insulin & Proinsulin. This assay employs an antibody specific for human Insulin coated on a 96-well plate. Standards and samples are pipetted into the wells and Insulin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Insulin antibody is added. After washing away unbound biotinylated antibody,HRP-conjugated streptavidin is pipetted to the well The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Insulin bound
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