Lipase Amylase Biochemistry Test Kit
Athenese DX Lipase Amylase Biochemistry Test Kit
The TRUEchemie Lipase liquid reagent test kit is used for the direct quantitative determination of LIPASE in human serum or plasma.
- Ready- to-use, Liquid stable reagents of 4:1 ratio
- Enzymatic Colorimetric method
- Linearity up to 250 U/L
- Ascorbic Acid up to 30 mg/dL Bilirubin up to 60 mg/dL, Hemoglobin 1000 mg/dL do not interfere
- Fixed time reaction with reaction time just 2 minutes
The pancreatic lipase in presence of colipase, desoxycholate and calcium ions, hydrolyses the substrate 1-2-O-dilauryl-rac-glycero-3- glutaric acid-(6′ -methylresorufin)-ester. The sequence of reactions involved in the enzymatic direct lipase determination is the following: 1-2-O-dilauryl-rac-glycero-3-glutaric-(6′ -methylresorufin)-ester Lipase −−−−−−→1-2-O-dilauryl-rac-glycerol + Glutaric-6′-methylresorufin-ester (unstable) −−−−−−−→Glutaric acid + Methylresorufin OH The rate of methylresorufin formation, measured photometrically, is proportional to the catalytic concentration of lipase present in the sample
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