Athenese DX URIC Acid Biochemistry Test Kit

The TRUEchemie Uric acid reagent test for the quantitative determination of Uric acid in human serum or plasma or urine.

• Ready- to-use, Liquid stable mono reagent
• Uricase -POD method with end point reaction
• Detection range of 0.03 mg/dL to 25 mg/dL
• BLANIL -6 stabilizers and Liposol incorporated
• Can be used in Calorimeters, semi and fully automated instruments

PRINCIPLE 

The enzymatic reaction sequence employed in the assay of uric acid is as follows  Uricase Uric acid + O2+ 2 H2O  Allantoin + CO2 + H2O2  Peroxidase 2 H2O2 + 4-Aminoantipyrine + DHBS   chromogen + 4 H2O  Uric acid is converted by uricase into allantoin and hydrogen peroxide. The hydrogen peroxide initiates the coupling of 4-aminoantipyrine to 3,5-dichloro-2-hydroxybenzene sulfonic acid (DHBS) to form the chromogen which is measured at 520 nm and which is proportional to the amount of hydrogen peroxide generated from uric acid.

KFT KIT Biochemistry Kit