Athenese DX ALT SGPT Biochemistry Test Kit

The TRUEchemie SGPT (ALT) Liquid Reagent(kinetic method)Test Kit is for the quantita tive determination of Serum Glutamate Pyruvate Transaminase (SGPT) or Alanine Aminotransferase (ALT) in human serum or plasma.

• Ready- to-use, Liquid stable reagents of 4:1 ratio
• NADH and enzymes specially stabilized
• IFCC method with real time kinetic reaction
• Detection range of 1.8 U/L to 500 U/L
• No inference of ≤ 40 mg/dL of Bilirubin and ≤ 2000 mg/dL of Triglyceride
• Results in just 4 minutes

PRINCIPLE  SGPT (ALT) L-Alanine 2-oxoglutarate  Pyruvate L-Glutamate        LDH Pyruvate NADH H    Lactate NAD H2O The pyruvate formed in the first reaction is reduced to lactate in the presence of lactate dehydrogenase and NADH. The activity of SGPT is determined by measuring the rate of oxidation of NADH at 340 nm. Endogenous sample pyruvate is converted to lactate by LDH during the lag phase prior to measurement. LFT Kit Biochemistry Kit