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subtotal : ₹ 00.00
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CTK Biotech, USA
Pack Size:30 Test/Kit
Leptospirosis, a bacterial disease caused primarily by Leptospira interrogans, can be challenging to diagnose due to its wide range of symptoms. The detection of Leptospira-specific antibodies, such as IgM and IgG, is crucial for accurate diagnosis. A variety of tests and kits are available to aid in the rapid diagnosis of leptospirosis, each with varying levels of sensitivity, specificity, and cost. The OnSite Leptospira IgG/IgM Combo Rapid Test is a popular choice for diagnosing leptospirosis due to its simplicity and quick results. This test uses a rapid test cassette, designed to detect both IgG and IgM antibodies in a patient's serum, plasma, or whole blood sample. The test procedure is straightforward: you simply add the sample to the designated well on the cassette and then add a few drops of the provided buffer. Within minutes, the results are displayed, indicating whether Leptospira antibodies are present. The Leptospira card test is another common rapid test that uses a card-based system to detect Leptospira antibodies. This test offers a quick and easy way to screen for leptospirosis. Like the rapid test cassette, this method involves adding a blood sample to the test card and then observing the results. For more precise diagnosis, the BEST DIAGNOSIS TEST for leptospirosis would be the gold standard test, typically the microscopic agglutination test (MAT). Although it provides highly accurate results, the MAT is more complex and time-consuming, requiring laboratory facilities and skilled personnel. When considering the cost of leptospira kits, the rapid test kits are generally more affordable than the MAT. This makes them suitable for use in resource-limited settings or for quick screening in clinical environments. However, while they are cost-effective and provide rapid results, they may have lower sensitivity compared to more complex tests like the MAT. The price of the leptospirosis test kit varies depending on the brand, the number of tests per kit, and the region. As with any diagnostic test, the choice of which kit to use depends on the required accuracy, budget, and available resources. In summary, rapid test kits such as the Leptospira IgG/IgM Combo Rapid Test and the Leptospira card test are valuable tools for the quick diagnosis of leptospirosis. While they offer convenience and cost-effectiveness, it's important to remember that for definitive diagnosis, more comprehensive testing like the MAT might be required.
The OnSite Leptospira IgG/IgM Combo Rapid Test is a lateral flow chromatographic immunoassay that can be performed within 15-20 minutes by minimally skilled personnel, and without the use of laboratory equipment. This test is intended to be used for the simultaneous detection and differentiation of IgG and IgM antibodies to Leptospira interrogans (L. interrogans) in human serum, plasma or whole blood. It is intended to be used as a screening test by professionals, and provides a preliminary test result to aid in the diagnosis of infection with L. interrogans. Any interpretation or use of this test result must also rely on other clinical findings as well as on the professional judgment of health care providers. Recommended for use:
Each kit contains:
TEST PRINCIPLE: The OnSite Leptospira IgG/IgM Combo Rapid Test is a lateral flow chromatographic immunoassay. The test cassette consists of: 1) a burgundy colored conjugate pad containing L. interrogans antigens conjugated with colloidal gold (Leptospira conjugates) and a control antibody conjugated with colloidal gold, 2) a nitrocellulose membrane strip containing two test lines (M and G lines) and a control line (C line). The M line is pre-coated with monoclonal anti-human IgM for the detection of IgM anti-L. interrogans, G line is pre-coated with monoclonal anti-human IgG for the detection of IgG antiL. interrogans, and the C line is pre-coated with a control line antibody. When an adequate volume of test specimen is dispensed into the sample well of the cassette, the specimen migrates by capillary action across the cassette. IgM anti-L. interrogans, if present in the specimen, will bind to the Leptospira conjugates. The immunocomplex is then captured on the membrane by the pre-coated anti-human IgM antibody forming a burgundy colored M line, indicating an IgM anti-L. interrogans positive test result. IgG anti-L. interrogans, if present in the specimen, will bind to the Leptospira conjugates. The immunocomplex is then captured on the membrane by the pre-coated antihuman IgG forming a burgundy colored G line, indicating an IgG anti-L. interrogans positive test result. Absence of any test lines (M and G) suggests a negative result. The test contains an internal control (C line) which should exhibit a burgundy colored line of the immunocomplex of the control antibodies regardless of color development on any of the test lines. If the C line does not develop, the test result is invalid and the specimen must be retested with another device.
