Athenese DX ADA Biochemistry Test Kit

The TRUEchemie ADA liquid reagent test kit is used for the direct quantitative determination of Adenosine Deaminase (ADA) in human serum or plasma.

• Ready-to-use, stable liquid reagent of 2:1 ratio
• Linearity up to 200 U/L
• Hemoglobin (≤ 800 mg/dL), Intralipid (≤ 1000 mg/dL) and ascorbic acid (≤ 50 mg/dL) do not interfere
• Packed with calibrator

PRINCIPLE  ADA kit is based on the enzymatic deamination of adenosine to inosine which is converted to hypoxanthine by purine nucleoside phosphorylase (PNP). Hypoxanthine is then converted to uric acid and hydrogen peroxide (H2O2) by xanthine oxidase (XOD). H2O2 is further reacted with N-Ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline (EHSPT) and 4-aminoantipyrine (4- AA) in the presence of peroxidase (POD) to generate quinone dye which is monitored in a kinetic manner. One unit of ADA is defined as the amount of ADA that generates one µmole of inosine from adenosine per min at 37°C. ADA Adenosine H2O-------- Inosine NH3 PNP Inosine Pi------ Hypoxanthine Ribose 1-phosphate, XOD  Hypoxanthine 2H2O 2O------- Uric acid 2H2O2    POD  2H2O2 4-AA TOOS------------- 4H2O Quinone dye Biochemistry Kit