J MITRA ANTI-D (IgM) Monoclonal Antibodies

INTENDED USE Anti-D (IgM) Monoclonal Antibodies are in-vitro culture supernatant of hybrids obtained by cellular fusion (Hybridoma Technology). It is a monoclonal antibody for Rh typing and is for in-vitro use only.

PRINCIPLE OF THE TEST

Human red cells are classified as Rh positive (Rh ) or Rh negative (Rh -) depending upon the presence or absence of ‘D’ antigen on them. Major percentage of the population is Rh positive. Human red blood cells possessing D antigen will agglutinate in the presence of corresponding antibody. Agglutination of red cells with Anti-D (IgM) Monoclonal antibodies indicates the presence of D-antigen and hence Rh positive result. It can detect D antigen (Rh₁) & high grade Du antigen (weaker variant of antigen D). Incase of no agglutination is obtained with Anti- D (IgM) Monoclonal antibodies, the cells should be then checked for the presence of low grade Du antigen by Coomb’s test using Anti-D (IgG) & Anti Human Globulin serum (Coomb’s Reagent).

STABILITY

The Antibodies are stable at 2-8°C for 18 months. blood group kits,blood grouping kit ,blood group,sera,antibodies blood group