Athenese DX Direct HDL Cholesterol Biochemistry Test Kit

The TRUEchemie Direct HDL cholesterol test kit for the “in vitro” quantitative determination of high-density lipoprote in cholesterol ( HDL-C) in human serum or plasma. Product Features:

• Ready-to-use, stable liquid reagent of 3:1 ratio
• Detection range of 2.5 – 200 mg/dL
• Hemoglobin (? 100 mg/dL), Bilirubin (? 20 mg/dL) were found to exhibit negligible interference (< 5 %)
• Packed with calibrator

PRINCIPLE  The HDL Cholesterol assay is a homogeneous method for directly measuring HDL-C concentrations in serum or plasma without the need for any off-line pretreatment or centrifugation steps. The method is in a two reagent format and depends on the properties of a unique detergent, as illustrated. This method is based on accelerating the reaction of cholesterol oxidase (CO) with non-HDL unesterified cholesterol and dissolving HDL selectively using a specific detergent. In the first reagent, non-HDL unesterified cholesterol is subject to an enzyme reaction and the peroxide generated is consumed by a peroxidase reaction with DSBmT yielding a colorless product. The second reagent consists of a detergent capable of solubilizing HDL specifically, cholesterol esterase (CE) and chromogenic coupler to develop color for the quantitative determination of HDL-C. This may be referred to as the Accelerator Selective Detergent methodology. Accelerator CO HDL, LDL, VLDL, ---------------------------------> Non-Reactive LDL,  Chylomicrons, DSBmT Peroxidase    VLDL, Chylomicrons, HDL Specific Detergent HDL ------------------------------------------------> HDL Disrupted, CE HDL Cholesterol --------------------------------> Δ4 Cholesterone H2O2,   CO H202 DSBmT 4-AAP ------------------------> Color Development   Peroxidase Biochemistry Kit