CTK Biotech, USA Free T3 ELISA Kit 96 Test

The RecombiLISA Free T3 ELISA Kit is competitive solid-phase enzyme-linked immunosorbent assay for the quantitative determination of free triiodothyronine (fT3) levels in human serum. A competitive solid-phase enzyme-linked immunoabsorbant assay for the quantitative determination of free triiodothyronine (fT3) levels in human serum.

• Sensitivity: 274 ± 0.077 pg/mL at 95% confidence level
• Specificity: <0.001 cross reactivity with l-thyroxine at 10 ?g/mL, iodotyrosine at 10 ?g/mL, diiodotyrosine at 10 ?g/mL, and d-thyroxine at 10 ?g/mL, respectively
• Accuracy: correlation coefficient 0.958 ± 0.087 at 95% confidence level
• Intra Assay Precision: CV < 5.1%
• Inter Assay Precision: CV < 7.5%
• Shelf life: 18 months
• Open kit stability: 8 weeks at 2-8 °C, or until the expiration date

• Anti-fT3 Ab Coated Microwells
• fT3 Calibrators C1-C6
• HRP-T3 Conjugate
• Wash Buffer (30X Concentrate)
• TMB Substrate
• Stop Solution
• ELISA Working Sheet

TEST PRINCIPLE  The RecombiLISA Free T3 ELISA Test is a competitive solid-phase enzyme-linked immunosorbent assay for the quantitative measurement of fT3 in human serum.The RecombiLISA Free T3 ELISA Test is comprised of two key components: 1) Solid microwells pre-coated with anti-fT3 antibody, 2) Liquid conjugate comprised of horseradish peroxidase conjugated with T3 (HRPT3 conjugate). During the assay, the test specimen and the HRP-T3 conjugate are simultaneously incubated with the microwells coated with anti-fT3 antibody. The free T3 in the patient specimen will then compete with the HRP-T3 conjugate for binding to the anti-fT3 antibodies coated on the microwell surface. The amount of conjugate bound to the microwell surface is inversely proportional to the concentration of fT3 in the patient specimen. Unbound conjugate are then removed by washing. The presence of the conjugate complex is shown by the development of a blue color upon the additional TMB substrate. The reaction is then terminated with Stop Solution and absorbance is determined using a spectrophotometer at 450/620-690 nm. The color intensity reflects the amount of bound HRP-T3 conjugate and is inversely proportional to the amount of fT3 in the patient specimen, within the dynamic range of the assayA standard curve is generated on graph paper by plotting the absorbance value for each fT3 calibrator measured at a wavelength of 450/620-690 nm on one axis, versus the concentration of the fT3 calibrators on the other, and drawing a best-fit line. The fT3 concentration in each specimen is then determined directly using this curve.