BIONEOVAN HAV IgM Elisa Kit 96Test

INTENDED USE Hepatitis a virus test elisa kits is ELISA Kit for IgM Antibody to Hepatitis A Virus is an in vitro enzyme immunoassay for the detection of HAV-IgM in human serum or plasma PRINCIPLE HAV elisa test kit uses capture ELISA method to detect anti-HAV IgM. The purified mouse anti-human IgM (μchain) antibody is coated on the solid phase of multi-wells. A conjugate HAV-Ag is added to the coated wells after diluted sample added and incubated. Then horseradish peroxides labeled HAV-Ag is added. If HAV-IgM is present in the sample, a complex of Anti-μ-chain-HAV-IgM –HAV-Ag -labeled with HRP will form. Wash wells to remove other unbounded serum components, incubate with substrate (TMB) to form a colored product, and measure the absorbance at 450nm to indicate the presence or absence of HAV-IgM in the sample. The test is special, sensitive, reproducible and easy to operate.