Athenese DX HIV 1 2 Ab ELISA kit 96 Test

The TRUSTwell HIV 1 2 Ab ELISA Kit is a solid phase enzyme linked immunoabsorbent assay for the qualitative detection of anti-HIV-1 including subtype O and anti-HIV-2 antibodies (including isotype IgG, IgM and IgA) in human serum or plasma.

• Based on double Ag-Sandwich immunoassay technique to ensure high specificity
• Solid microwells pre-coated with recombinant HIV-1 and HIV-2 Ag
• Liquid conjugates composed of recombinant HIV-1 and HIV-2 Ag conjugated with HRP
• Relative sensitivity: 100% and specificity: 99.9 %
• Comparable accuracy to the leading other ELISA kits
• Clear borderlines with high accuracy positive and negative controls
• Breakaway microwell strips
• Evaluated by NIB
• Assay time: 90 Minutes
• 12-months shelf life at 2-8 °C
• Microwells coated with HIV-1 & HIV-2 antigen

• HIV Ab positive control
• HIV Ab negative control
• Sample diluent
• HIV 1 2 HRP conjugates
• Wash buffer (30 x concentrate)
• TMB substrate A
• TMB substrate B
• Stop solution
• ELISA Working Sheet
• Product insert

TEST PRINCIPLE  TRUSTwell HIV 1 2 Ab ELISA Kit is a solid phase enzyme linked immunoabsorbent assay based on the principle of the double antigen-sandwich technique for the detection of the various antibodies against HIV-1 and/or HIV-2 in human serum or plasma. The TRUSTwell HIV 1 2 Ab ELISA Kit  is composed of two key components: 1) Solid microwells pre-coated with recombinant HIV-1 and HIV-2 antigens; 2) Liquid conjugates composed of recombinant HIV-1 and HIV-2 antigens conjugated with horse radish peroxidase (HIV 1 2 HRP conjugates). During the assay, the test specimen is first incubated with the coated microwells.  The anti-HIV-1 and anti-HIV-2 antibodies, if present in the specimen, bind to the antigens coated on the microwell surface.In the second incubation with the HRP- HIV 1 2 conjugates, the anti-HIV-1 and antiHIV-1 antibodies absorbed on the surface of microwell react to the HRP-HIV 1 2 conjugates.  Unbounded conjugates are then removed by washing. The presence of the complexed conjugates is shown by a blue color upon additional incubation with TMB substrate. The reaction is stopped with Stop Solution and absorbance are read using a spectrophotometer at 450 /620-690 nm.