Athenese DX HIV Ag-Ab ELISA Kit 96 Test

The TRUSTwell HIV Ag-Ab ELISA Kit is a solid phase enzyme linked immunoabsorbent assay for the qualitative detection of HIV-1 P24 antigen, anti-HIV-1 including subtype O and anti-HIV-2 antibodies (including isotype IgG, IgM and IgA) in human serum or plasma.

• Based on double Ag/Ab-Sandwich immunoassay technique to ensure high specificity

• Solid microwells pre-coated with recombinant HIV-1, HIV-2 Ag and Anti-P24 Ab
• Liquid conjugates composed of recombinant HIV-1, HIV-2 Ag & SA conjugated with HRP
• Relative sensitivity: 100% and specificity: 99.9 %
• Comparable accuracy to the leading other ELISA kits
• Clear borderlines with high accuracy positive and negative controls
• Breakaway microwell strips
• Evaluated by NIB
• Assay time: 90 Minutes
• 12-months shelf life at 2-8 °C

• Microwells coated with anti-P24 Ab, recombinant HIV-1 and HIV-2 antigen
• Biotinylated P24 Ab
• HIV Ag positive control
• HIV Ag negative control
• HIV negative control
• HRP–avidin, HIV 1 2 conjugate mixture
• Sample diluent
• Wash buffer (30 x concentrate)
• TMB substrate A
• TMB substrate B
• Stop solution
• ELISA Working Sheet
• Product insert

TEST PRINCIPLE  The TRUSTwell HIV Ag-Ab ELISA Kit is a solid phase enzyme linked immunoabsorbent assay based on the principle of the double antigen/antibody sandwich technique for the detection of the various antibodies against HIV-1 and/or HIV-2 and HIV antigen in human serum or plasma. The TRUSTwell HIV Ag-Ab ELISA Kit is composed by two systems: 1) Solid microwells pre-coated with  Recombinant HIV-1 and HIV-2 antigens and anti-P24 antibody 1) Liquid conjugates composed of  a. Recombinant HIV-1 and HIV-2 antigens conjugated with horse reddish peroxidase (HRP-HIV1 2 conjugates) and avidin conjugated with HRP (HRP-avidin conjugates) b. Anti-P24 antibody conjugated with biotin (Biotinylated P24 Ab) During the assay, the test specimen is first incubated with the coated microwells and biotinylated P24 Ab.  The anti-HIV-1 and anti-HIV-2 antibodies, if present in the specimen, bind to the antigens coated on the microwell surface.  At meanwhile, P24, if present in the specimen, binds to the biotinylated P24 Ab in the solution and the anti-P24 antibody on the microwell surface.In the second incubation with the HRP-HIV 1 2 and HRP-avidin conjugates, the above bounded anti-HIV-1 and anti-HIV-2 antibodies and bounded P24 antigen reactive to the HRP conjugates, forming HRP-complex.Unbounded conjugates are then removed by washing. The presence of the complexed conjugates is shown by a blue color upon additional incubation with TMB substrate. The reaction is stopped with Stop Solution and absorbances are read using a spectrophotometer at 450 /620-690 n