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TSH Quanti Card is a sensitive immunoassay for the quantitative determination of Thyroid Stimulating Hormone (TSH) in human serum/ plasma with i-Quant Analyzer. PRINCIPLE (ANTIGEN-ANTIBODY REACTION) TSH Quanti Card is based on principle of fluorescence sandwich immunoassay technology. The test uses anti TSH antibody immobilized on a nitrocellulose strip. When a sample is added to the cartridge, TSH antigen from the sample will form a complex with another specific TSH antibodies conjugated to fluorochrome. On addition of assay buffer, this complex migrates through the nitrocellulose strip by capillary action. When the complex meets the line of the corresponding specific antibody immobilized on nitrocellulose membrane, the fluorescence signal is produced. This signal is interpreted and the result is displayed on iQuant Analyzer in form of results.
