Athenese DX Alpha Amylase Biochemistry Test Kit

The TRUEchemie α – Amylase (Alpha amylase) test kit is for the quantitative determination of Amylase concentration in human serum or plasma.

• Ready-to-use, single reagent
• Linearity up to 2000 U/L
• Bilirubin (mixed isomers) < 10 % interference up to 600 µmol/L, haemolysis < 10 % interference up to 5 g/L and lipemia < 10 % interference up to 5 g/L
• Factor or calibrator-based assay

PRINCIPLE  The direct amylase assay involves the use of a chromogenic substrate CNPG3 (2-chloro-4nitrophenyl linked with Galactomaltoside) which acts upon α - Amylase to release more than 95% of 2-chloro-4-nitrophenol (CNP), and form 2-chloro-4-nitrophenyl-α-D-maltoside (CNPG2), maltotriose (G3) and Glucose (G). The rate of formation of 2-chloro-4-nitrophenol is proportional to the α - Amylase activity in the sample, which can be monitored by kinetic assay at 405 nm. α- Amylase 10 CNPG3, 9CNP CNPG2 9G3 G Biochemistry Kit