Athenese DX Lipase Amylase Biochemistry Test Kit

The TRUEchemie Lipase liquid reagent test kit is used for the direct quantitative determination of LIPASE in human serum or plasma.

• Ready- to-use, Liquid stable reagents of 4:1 ratio
• Enzymatic Colorimetric method
• Linearity up to 250 U/L
• Ascorbic Acid up to 30 mg/dL Bilirubin up to 60 mg/dL, Hemoglobin 1000 mg/dL do not interfere
• Fixed time reaction with reaction time just 2 minutes

PRINCIPLE  The pancreatic lipase in presence of colipase, desoxycholate and calcium ions, hydrolyses the substrate 1-2-O-dilauryl-rac-glycero-3- glutaric acid-(6' -methylresorufin)-ester. The sequence of reactions involved in the enzymatic direct lipase determination is the following: 1-2-O-dilauryl-rac-glycero-3-glutaric-(6' -methylresorufin)-ester    Lipase −−−−−−→1-2-O-dilauryl-rac-glycerol Glutaric-6'-methylresorufin-ester (unstable) −−−−−−−→Glutaric acid Methylresorufin OH The rate of methylresorufin formation, measured photometrically, is proportional to the catalytic concentration of lipase present in the sample Biochemistry Kit