NOVATEC, Germany
Pack Size:96 Test/Kit
Enzyme-Linked Immunosorbent Assay (ELISA) is a common diagnostic method used to detect antibodies, such as IgM and IgG, that indicate infection with Leptospira interrogans. The Leptospirosis ELISA Diagnostic Kit allows for sensitive detection of these antibodies in a patient's serum, plasma, or whole blood. This kit, including the Lepto IgM ELISA Test, is designed to aid in diagnosing leptospirosis, providing reliable results when assessing for recent or ongoing infection. The Principle of ELISA Leptospirosis Kit revolves around antibody-antigen reactions. The test uses a specific antigen from Leptospira to capture corresponding antibodies in the sample. After washing to remove unbound antibodies, an enzyme-linked secondary antibody is applied. Upon adding a substrate, the reaction produces a detectable signal, indicating the presence of the target antibody. The Leptospira IgG ELISA Kit and the Leptospira IgM ELISA Panbio are designed to detect specific antibody classes, providing insight into the stage of the infection. While IgM antibodies indicate a recent infection, IgG antibodies suggest a past infection or ongoing recovery. This makes the Leptospira antibody IgM ELISA Test especially useful for early diagnosis of leptospirosis. Though ELISA tests are widely used, the gold standard ELISA TEST FOR LEPTOSPIRA is the microscopic agglutination test (MAT), known for its high specificity and accuracy. Despite this, the ELISA tests are more accessible for rapid diagnosis in clinical settings. When using a leptospira pathology diagnostic test kit, medical professionals should consider the sensitivity, specificity, and the kit's ease of use. ELISA-based tests offer a balance of accuracy and practicality, making them a preferred choice for diagnosing leptospirosis.
INTENDED USE The Leptospira IgG ELISA is intended for the qualitative determination of IgG class antibodies against Leptospira spp. in human serum or plasma (heparin). Test Description The qualitative immunoenzymatic determination of specific antibodies is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique.Microplates are coated with specific antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material a horseradish peroxidase (HRP) labelled conjugate is added.This conjugate binds to the captured antibodies. In a second washing step unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product.The intensity of this product is proportional to the amount of specific antibodies in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450/620 nm is read using an ELISA microwell plate reader