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subtotal : ₹ 00.00
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CTK Biotech, USA
Pack Size:96 Test/Kit
Enzyme-Linked Immunosorbent Assay (ELISA) is a common diagnostic method used to detect antibodies, such as IgM and IgG, that indicate infection with Leptospira interrogans. The Leptospirosis ELISA Diagnostic Kit allows for sensitive detection of these antibodies in a patient's serum, plasma, or whole blood. This kit, including the Lepto IgM ELISA Test, is designed to aid in diagnosing leptospirosis, providing reliable results when assessing for recent or ongoing infection. The Principle of ELISA Leptospirosis Kit revolves around antibody-antigen reactions. The test uses a specific antigen from Leptospira to capture corresponding antibodies in the sample. After washing to remove unbound antibodies, an enzyme-linked secondary antibody is applied. Upon adding a substrate, the reaction produces a detectable signal, indicating the presence of the target antibody. The Leptospira IgG ELISA Kit and the Leptospira IgM ELISA Panbio are designed to detect specific antibody classes, providing insight into the stage of the infection. While IgM antibodies indicate a recent infection, IgG antibodies suggest a past infection or ongoing recovery. This makes the Leptospira antibody IgM ELISA Test especially useful for early diagnosis of leptospirosis. Though ELISA tests are widely used, the gold standard ELISA TEST FOR LEPTOSPIRA is the microscopic agglutination test (MAT), known for its high specificity and accuracy. Despite this, the ELISA tests are more accessible for rapid diagnosis in clinical settings. When using a leptospira pathology diagnostic test kit, medical professionals should consider the sensitivity, specificity, and the kit's ease of use. ELISA-based tests offer a balance of accuracy and practicality, making them a preferred choice for diagnosing leptospirosis. For accurate diagnosis of leptospira, CTK Biotech Leptospira IgM ELISA Kit 96 Test is the best and most reliable.
The RecombiLISA Leptospira IgM ELISA Kit is an indirect, solid phase enzyme-linked immunosorbent assay. The two main kit components are: 1) A 96 breakable well plate with the solid microwells pre-coated with L. interrogans antigen. 2) Liquid conjugates composed of monoclonal anti-human IgM reagent conjugated with horseradish peroxidase (HRP-anti-human IgM conjugates). This test is intended to be used for the qualitative detection of IgM anti-Leptospira interrogans (L. interrogans) in human serum or plasma. It is intended to be used by professionals as an aid in the diagnosis of acute infection with L. interrogans. Any interpretation or use of this test result must also rely on other clinical findings as well as on the professional judgment of health care providers. Recommended for use:
TEST PRINCIPLE The RecombiLISA Leptospira IgM ELISA Kit is a solid phase enzyme linked immunosorbent assay based on the principle of the indirect immunoassay technique for the qualitative detection of IgM anti-L. interrogans in human serum or plasma. The RecombiLISA Leptospira IgM ELISA Kit is composed of two key components: 1) Solid microwells pre-coated with L. interrogans antigen; 2) Liquid conjugates composed of monoclonal anti-human IgM reagent conjugated with horseradish peroxidase (HRP-anti-human IgM conjugates). During the assay, the test specimen is firstly incubated with the coated microwells. The IgM anti-L. interrogans, if present in the specimen, reacts to the antigen coated on the microwell surface. In the second incubation with the HRP-anti-human IgM conjugates, the IgM anti-L. interrogans antibody adsorbed on the surface of the microwells reacts to the conjugates, forming an immunocomplex. Unbound conjugates are then removed by washing. The presence of the conjugate complex is shown by development of a blue color upon additional incubation with TMB substrate. The reaction is stopped with Stop Solution and the absorbance is determined using a spectrophotometer at 450/620-690 nm. OD values must be measured within 5 minutes after the addition of the Stop Solution
